Composite

Part:BBa_K3578010:Design

Designed by: Ruyi Shi   Group: iGEM20_Worldshaper-Nanjing   (2020-09-20)


AnGlu Starch degradation


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3683
    Illegal EcoRI site found at 3733
    Illegal EcoRI site found at 4695
    Illegal SpeI site found at 756
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3683
    Illegal EcoRI site found at 3733
    Illegal EcoRI site found at 4695
    Illegal NheI site found at 817
    Illegal SpeI site found at 756
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3683
    Illegal EcoRI site found at 3733
    Illegal EcoRI site found at 4695
    Illegal BglII site found at 2537
    Illegal BglII site found at 5964
    Illegal BamHI site found at 3157
    Illegal XhoI site found at 4678
    Illegal XhoI site found at 4707
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3683
    Illegal EcoRI site found at 3733
    Illegal EcoRI site found at 4695
    Illegal SpeI site found at 756
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3683
    Illegal EcoRI site found at 3733
    Illegal EcoRI site found at 4695
    Illegal SpeI site found at 756
    Illegal NgoMIV site found at 3821
    Illegal AgeI site found at 3364
    Illegal AgeI site found at 5292
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BsaI and SapI restriction enzymes were not present in this cassette.


Source

EXP, XPR2t were amplified from Yarrowia lipolytica. Leu marker was synthesized chemically by TSINGKE Biotech Co., Ltd., China. AnGlu was codon-optimized and synthesized chemically by TSINGKE Biotech Co., Ltd., China.

References