Composite
Part:BBa_K3578010:Design
Designed by: Ruyi Shi Group: iGEM20_Worldshaper-Nanjing (2020-09-20)
AnGlu Starch degradation
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 3683
Illegal EcoRI site found at 3733
Illegal EcoRI site found at 4695
Illegal SpeI site found at 756 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 3683
Illegal EcoRI site found at 3733
Illegal EcoRI site found at 4695
Illegal NheI site found at 817
Illegal SpeI site found at 756 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 3683
Illegal EcoRI site found at 3733
Illegal EcoRI site found at 4695
Illegal BglII site found at 2537
Illegal BglII site found at 5964
Illegal BamHI site found at 3157
Illegal XhoI site found at 4678
Illegal XhoI site found at 4707 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 3683
Illegal EcoRI site found at 3733
Illegal EcoRI site found at 4695
Illegal SpeI site found at 756 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 3683
Illegal EcoRI site found at 3733
Illegal EcoRI site found at 4695
Illegal SpeI site found at 756
Illegal NgoMIV site found at 3821
Illegal AgeI site found at 3364
Illegal AgeI site found at 5292 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
BsaI and SapI restriction enzymes were not present in this cassette.
Source
EXP, XPR2t were amplified from Yarrowia lipolytica. Leu marker was synthesized chemically by TSINGKE Biotech Co., Ltd., China. AnGlu was codon-optimized and synthesized chemically by TSINGKE Biotech Co., Ltd., China.